This is a preview of subscription written content, log in via an institution to check entry. Obtain this short articleTo reduce these difficulties we spot a guard column prior to the analytical column. A Guard column normally includes the identical particulate packing content and stationary phase as the analytical column, but is significantly short
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Substantial-efficiency liquid chromatography (HPLC) will involve the injection of a little volume of liquid sample right into a tube filled with tiny particles (3 to five microns (µm) in diameter known as the stationary section) where particular person components of the sample are moved down the packed tube having a liquid (cellular section) compe